Suicide gene therapy for plasma cell tumors

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Suicide gene therapy for plasma cell tumors.

Suicide gene therapy for plasma cell tumors was attempted in severe combined immunodeficient (SCID) mice injected with human myeloma cell lines. Initially, a ganciclovir-induced bystander effect was observed in vitro using myeloma cells transduced with a herpes simplex thymidine kinase (HSVtk) gene. Transduced cells injected subcutaneously (SC) into SCID mice could be eradicated by the administ...

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Cancer is now an important target for gene therapy. Most animal experiments were done with the use of transplanted tumors produced by implantation of cell lines, an approach that ignores histologic and anatomic specificity of organs. Chemically induced orthotopic models are much superior to the subcutaneous transplanted models with respect to the evaluation of the efficiency of gene transfer an...

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This review focuses on the prodrugs used in suicide gene therapy. These prodrugs need to satisfy a number of criteria. They must be efficient and selective substrates for the activating enzyme, and be metabolized to potent cytotoxins preferably able to kill cells at all stages of the cell cycle. Both prodrugs and their activated species should have good distributive properties, so that the resu...

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ژورنال

عنوان ژورنال: Blood

سال: 1996

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v88.6.2192.bloodjournal8862192